Acute Toxicity Test of Mulberry Leaf Extract (Morus rubra L) as a Basic Ingredient of Candida Albicans Antifungal on Rattus Norvecigus
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https://doi.org/10.36456/embrio.v16i1.9028Keywords:
Acute toxicity, Mulberry leaf extract, Candida albicans, LD50Abstract
Previous research on the effectiveness of mulberry leaves in the growth of candida albicans showed that C. albicans is inhibited in vitro. Mulberry leaf extract is used as a basic ingredient for treatment. In addition to being tested for effectiveness, testing is needed to ensure the level of acute toxicity in experimental animals in determining toxicity levels. The study aimed to test the acute toxicity of mulberry leaf extract on Rattus Norvecigus media in determining the level of toxicity before becoming the basic ingredient of VVC antifungal. The research method uses an experimental laboratory post-test-only control group design with a 9 Rattus Norvegicus media. The average dose is not lethal using LD50 values. Research analysis using ANOVA is One Way to determine the difference in the average weight change of experimental animals. The study's results did not show any deaths of experimental animals or toxic symptoms in administering mulberry leaf extract doses of 0 mg, 100mg, 1000 mg, 1600mg, 2900mg, 5000mg. LD50 value more than 8g/kg BW, included in the non-toxic category (5-15 g/kg BW). The ANOVA statistical test showed a P-value of 0.781 more than a P-value of 0.05, that is, there was no difference in the average change in body weight in the experimental animal group given different treatment doses in each group. The research concludes that mulberry leaf extract does not show a lethal dose of acute toxicity, so it is safe to use as a basic ingredient in treatment sourced from natural ingredients.
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Bharani, S. E. R., Asad, M., Dhamanigi, S. S., & Chandrakala, G. K. (2010). Immunomodulatory activity of methanolic extract of Morus alba Linn. (mulberry) leaves. Pakistan Journal of Pharmaceutical Sciences, 23(1), 63–68.
Bitew, A., & Abebaw, Y. (2018). Vulvovaginal candidiasis: Species distribution of Candida and their antifungal susceptibility pattern. BMC Women’s Health, 18(1), 1–10. https://doi.org/10.1186/s12905-018-0607-z DOI: https://doi.org/10.1186/s12905-018-0607-z
BPOM RI. (2022). Peraturan Badan Pengawas Obat Dan Makanan Nomor 10 Tahun 2022 Tentang Pedoman Uji Toksisitas Praklinik Secara In Vivo. Badan Pengawas Obat Dan Makanan Republik Indonesia, 1–220. https://jdih.pom.go.id/view/slide/8a9d3b1b7a12d82cf9d09188339ef1ba/1379/10/2022
Dimitrijevic, D., Kostic, D., Paunovic, D., Mitic, M., Krstic, J., Misic, I. R., & Arsic, B. (2022). Antimicrobial Activity And The Quantitative Analyses Of Phenolic Compounds And Heavy Metals Of Red Mulberry Extracts (Morus Rubra L.) From Serbia. Studia Universitatis Babes-Bolyai. Chemia, 67, 195-207. https://doi.org/10.24193/subbchem.2022.1.13 DOI: https://doi.org/10.24193/subbchem.2022.1.13
Djohan, V., Angora, K. E., Vanga-Bosson, A. H., Konaté, A., Kassi, K. F., Kiki-Barro, P. C. M., Bedia-Tanoh, A. V., Miezan, S., Menan, E. I. H., & Yavo, W. (2019). Recurrent vulvo-vaginal candidiasis in Abidjan (Côte d’Ivoire): Aetiology and associated factors. Journal de Mycologie Medicale, 29(2), 127–131. https://doi.org/10.1016/j.mycmed.2019.04.002 DOI: https://doi.org/10.1016/j.mycmed.2019.04.002
Farr, A., Effendy, I., Frey Tirri, B., Hof, H., Mayser, P., Petricevic, L., Ruhnke, M., Schaller, M., Schaefer, A. P. A., Sustr, V., Willinger, B., & Mendling, W. (2021). Guideline: Vulvovaginal candidosis (AWMF 015/072, level S2k). Mycoses, 64(6), 583–602. https://doi.org/10.1111/myc.13248 DOI: https://doi.org/10.1111/myc.13248
Hafid, M., & Rahayu, R. (2022). Uji toksisitas akut (ld 50 ) ekstrak etanol daun gedi merah (Abelmoschus Manihot) Asal Sorong Selatan Terhadap Mencit (Mus Musculus). Fito Medicine: Journal Pharmacy and Sciences, 13(2), 84–87. https://doi.org/10.47650/fito.v13i2.430
Hidayatunnikmah, N., Latifah, A., Rosyida, D. A. C., & Safitri, S. D. (2022). Aktivitas Antimikroba Ekstrak Daun Mulberry ( Morus Rubra L ) Terhadap Penghambatan Pertumbuhan Jamur Candida Albicans-In Vitro. JIK (Jurnal Ilmu Kesehatan), 6(1), 175–183. http://dx.doi.org/10.33757/jik.v6i1.513 DOI: https://doi.org/10.33757/jik.v6i1.513
Khan, N. H. (2021). “MORUS RUBRA L: “Phytochemical Screening, Anti- Bacterial, Antioxidant and Anti-Hyperglycemic Activity Determination.” Biomedical Journal of Scientific & Technical Research, 39(4), 31443–31457. https://doi.org/10.26717/bjstr.2021.39.006320 DOI: https://doi.org/10.26717/BJSTR.2021.39.006320
Latifah, A., Hidayatunnikmah, N., & Safitri, S. D. (2022). Flavonoid Ekstrak Daun Mulberry Terhadap Daya Hambat Pertumbuhan Candida Albicans. SNHRP, 4, 807–816. https://snhrp.unipasby.ac.id/prosiding/index.php/snhrp/article/view/390
Lírio, J., Giraldo, P. C., Amaral, R. L., Sarmento, A. C. A., Costa, A. P. F., & Goncalves, A. K. (2019). Antifungal (oral and vaginal) therapy for recurrent vulvovaginal candidiasis: A systematic review protocol. BMJ Open, 9(5). https://doi.org/10.1136/bmjopen-2018-027489 DOI: https://doi.org/10.1136/bmjopen-2018-027489
Loomis TA. (1987). Essential of toxicology. 3rd ed.
Marrazzo, J. (2002). Vulvovaginal candidiasis. Clinical Evidence, 6(7), 1784–1796. https://doi.org/10.1177/1755738013479944 DOI: https://doi.org/10.1177/1755738013479944
Memete, A. R., Timar, A. V., Vuscan, A. N., Miere, F., Venter, A. C., & Vicas, S. I. (2022). Phytochemical Composition of Different Botanical Parts of Morus Species, Health Benefits and Application in Food Industry. Plants, 11(2). https://doi.org/10.3390/plants11020152 DOI: https://doi.org/10.3390/plants11020152
Miljković, V., Nikolić, G., Mihajlov-Krstev, T. M., & Arsić, B. (2018). Antibacterial Activities of Fruits Extracts of Three Mulberry Species (Morus Alba L., Morus Rubra L. and Morus Nigra L.) and Bilberry (Vaccinium Myrtillus L.). Acta Medica Medianae. 57(3), 5–12. https://doi.org/10.5633/amm.2018.0301 DOI: https://doi.org/10.5633/amm.2018.0301
Moshfeghy, Z., Tahari, S., Janghorban, R., & Sayadi, M. (2020). Association of sexual function and psychological symptoms including depression, anxiety and stress in women with recurrent vulvovaginal candidiasis. Journal of the Turkish German Gynecological Association, 21(2), 90–96. https://doi.org/10.4274/jtgga.galenos.2019.2019.0077 DOI: https://doi.org/10.4274/jtgga.galenos.2019.2019.0077
Sobel, J. D., Faro, S., Force, R. W., Foxman, B., Ledger, W. J., Nyirjesy, P. R., Reed, B. D., & Summers, P. R. (1998). Vulvovaginal candidiasis: Epidemiologic, diagnostic, and therapeutic considerations. American Journal of Obstetrics and Gynecology, 178(2), 203–211. https://doi.org/10.1016/S0002-9378(98)80001-X DOI: https://doi.org/10.1016/S0002-9378(98)80001-X
Swari, D. A. M. A., Santika, I. W. M., & Aman, I. G. M. (2020). Antifungal Activities Of Ethanol Extract Of Rosemary Leaf (Rosemarinus officinalis L.) AGAINST Candida albicans. Journal of Pharmaceutical Science and Application, 2(1), 28. https://doi.org/10.24843/jpsa.2020.v02.i01.p05 DOI: https://doi.org/10.24843/JPSA.2020.v02.i01.p05
Talapko, J., Juzbašić, M., Matijević, T., Pustijanac, E., Bekić, S., Kotris, I., & Škrlec, I. (2021). Candida albicans-the virulence factors and clinical manifestations of infection. Journal of Fungi, 7(2), 1–19. https://doi.org/10.3390/jof7020079 DOI: https://doi.org/10.3390/jof7020079
Thiriloshani, P., & Bharti, O. (2018). Antimicrobial activity of plant phenols from Chlorophora excelsa and Virgilia oroboides. African Journal of Biotechnology, 12(17), 2254–2261. https://doi.org/10.5897/ajb12.961 DOI: https://doi.org/10.5897/AJB12.961
Willems, H. M. E., Ahmed, S. S., Liu, J., Xu, Z., & Peters, B. M. (2020). Vulvovaginal Candidiasis: A Current Understanding and Burning Questions. Journal of fungi (Basel, Switzerland), 6(1), 27. https://doi.org/10.3390/jof6010027. DOI: https://doi.org/10.3390/jof6010027
Workowski, K. A., Bachmann, L. H., Chan, P. A., Johnston, C. M., Muzny, C. A., Park, I., Reno, H., Zenilman, J. M., & Bolan, G. A. (2021). Sexually Transmitted Infections Treatment Guidelines, 2021. MMWR Recommendations and Reports, 70(4), 1–187. https://doi.org/10.15585/mmwr.rr7004a1 DOI: https://doi.org/10.15585/mmwr.rr7004a1
Yang, Y., Shen, C., Li, J., Yuan, J., Yang, M., Wang, F., Li, G., Li, Y., Xing, L., Peng, L., Wei, J., Cao, M., Zheng, H., Wu, W., Zou, R., Li, D., Xu, Z., Wang, H., Zhang, M., … Liu, Y. (2020). Exuberant elevation of IP-10, MCP-3 and IL-1ra during SARS-CoV-2 infection is associated with disease severity and fatal outcome. MedRxiv, 2(December 2019). https://doi.org/10.1101/2020.03.02.20029975 DOI: https://doi.org/10.1101/2020.03.02.20029975
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