EFEKTIFITAS INDUKSI LASERPUNKTUR DAN OVAPRIM TERHADAP KECEPATAN PEMIJAHAN DAN JUMLAH TELUR YANG TERBUAHI PADA INDUK LELE (Clarias sp)

Dyah Hariani; Pungky Slamet Wisnu Kusuma

doi:10.36456/stigma.vol9.no2.a330

Authors

Dyah Hariani
Pungky Slamet Wisnu Kusuma

DOI:

https://doi.org/10.36456/stigma.vol9.no2.a330

Abstract

This study aimed to test the speed of spawning and the number of fertilized eggs at the parent catfish (Clarias sp) post-induction laserpuncture and ovaprim. This research was conducted at the Laboratory of Biology, University of PGRI Adi Buana Surabaya. The experimental design used was completely randomized design. The study consisted of three treatments, induction laserpuncture in reproductive acupoint precisely at 2/3 of the ventral body for 15 seconds, giving ovaprim 0.4 ml/kg body weight and control (without induction laserpuncture and without giving ovaprim), each repeated six times. Total catfish male parent used as many as 18 heads and parent female catfish as many as 18 heads of all the conditions are ripe gonads. Number of pools that use a total of 18 pools (for spawning catfish). The data collected is spawning catfish speed and the number of fertilized eggs. The results showed that the induction of laserpuncture precisely at 2/3 the ventral body for 15 seconds reproductive acupoint fastest spawning effect and eggs fertilization hight significant than ovaprim and control. Keywords: Induction laserpuncture, ovaprim, eggs fertilization, speed spawning catfish

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References

Azuadi, N.M., S. Siraj., .K. Daud, 1.A. Christianu.s, A. Harmin., Sungan and R. Britin. 2011. Enhancing ovulation of Malaysian Mahseer (Tor tambroides) in captivity by removal of dopaminergic inhibition. J.Fish. Aquat.Sci., 6(7):740-750.

Gadissa, S. and L. P Devi.2013.Evaluation of spawning induction of African catfish (Clarias gariepinus) by heteroplastic hypophysation. International Journal of Fisheries and Aquatic Studies.1(1): 22-25.

Hariani, D. 2015. Kombinasi Level Protein Dalam Pakan Induk dan Induksi Laserpunktur Untuk Meningkatkan Kualitas Telur Ikan Lele (Clarias sp). Disertasi. Pascasarjana Fakultas Perikanan dan Ilmu Kelautan. Universitas Brawijaya, Malang.

Hariani, D. and P.S.W. Kusuma. 2009. Biostimuli reproduksi ikan lele Dumbo (Clarias gariepinus) betina dengan penembakan laserpunktur. Jurnal Berkala Penelitian Hayati. PBI Cabang Jawa Timur. Edisi Khusus. 01 Desember 2009, 3D:79-83.

Hariani, D., A. P. W. Marhendra., Aulanni ‘am. and E. Suprayitno. 2014. Profile of catfish (Clarias sp) oocyte exposed by laserpuncture. Journal of Biology and Life Science, 5(2). 9 pp. ISSN 2157-6076.

Hariani, D., P.S.W. Kusuma, dan M.S. Widodo, 2010. Pemberdayaan kelompok pembenih lele untuk peningkatan produksi benih menggunakan laserpunktur sebagai upaya peningkatan pendapatan di desa Krecek,Kecamatan Pare, Kabupaten Kediri. Jurnal Aksi, 12(2):80-88.

Ingram, B.A., S. Sungan, G.J. Gooley, Y.S. Sim, D. Tinggi and S.S. De Silva, 2005. Induced spawning, larval development and rearing of two indigenous Malaysian mahseer, Tor tambroides and T. douronensis. Aquacult. Res, 36: 983-995.

J.Mittelmark and A. Kapuscinski. 2008. Induced reproduction in fish. Minnesota sea Grant. University of Minnesota.

Karu, T. I. 2000. Chapter IV. Cellular Mechanisms of Low Power Laser Therapy. Pp 79-100. Institute on Problems of Laser and Informatic Technologies of Russian Academy of Sciences. http://www. mededge. inc.com/nquest.pdf. Diakses 6 Maret 2011.

Kiran,B. R 1., K.S Murthy.* and M. Venkateshwarlu.2013. A review on induced breeding of cat fishes, murrels and climbing perches in India. Adv. Appl. Sci. Res, 4(4):310-323.

Kiran,B. R., K. S. Murthy . and M. Venkateshwarlu. 2013. A review on induced breeding of cat fishes, murrels and climbing perches in India. Adv.Appl.Sci.Res.,4(4):310-323.

Koutna, M. 2003. Response of microtubules of therapeutic laser irradiation. Cells, III:177-179.

Kusuma, P. S. W ., D. Hariani., A. T. Mukti. dan W. A. Satyantini. 2007. "Aplikasi Teknologi Laser untuk Peningkatan Produksi Lele dalam Rangka Pengembangan Ekonomi Masyarakat Ekonomi Masyarakat Desa di Kabupaten Boyolali Jawa Tengah," LP3K Kabupaten Boyolali, Boyolali.

Kusuma, P.S.W. 2013. Mekanisme pelepasan hormon gonadotropin ikan lele (Clarias sp) setelah dipapar laserpunktur pada titik reproduksi. Disertasi. Pascasarjana Fakultas Perikanan dan Ilmu Kelautan. Universitas Brawijaya, Malang.

Kusuma, P.S.W., N. Ngadiani and D. Hariani. 2015. Utilization of laserpuncture induction as spawning stimulation in catfish (Clarias sp.) cross breeding toward egg quality. Egyptian Journal of Aquatic Research, 41:353–358.

Kusuma, P.S.W., Agung P.M. Marhendra., Aulanni’am and Marsoedi. 2012. Mechanism of gonadotropin hormone release in catfish (Clarias sp) upon laserpuncture exposure to reproduction acupoint. International Journal of Basic and Applied Sciences IJBAS-IJENS. December. 2012, 12(06):177-182

Haniffa., T. Merlin. And J.S. Mohamed. 2000. Induced spawning of the striped murrel Channa striatvs using pituitary extracts, human chorionic gonadotropin, luteinizing hormone releasing hormone analogue and ovaprim. Act Icht.Piscat. 30(1):53-60., Ann.

Madu, C.T., C.C. Okwuego and C. Wonah. 2004. Dietary protein requirements of male and female broodstock: an economic factor for increased sustainability of private catfish hatcheries. In: 18th Annual Conference of the Fisheries Society of Nigeria (FISON) pp 67-77. 8-12 December, 2003. Owerri, Nigeria.

Marimuthu,K., N. Satthiyasilan., M. A. Rahman., A. Arshad., M. G. Raj. and J.Arockiaraj. 2015. Induced ovulation and spawning of African catfish Clarias gariepinus (Bloch) using ovaprim. Journal of Environment and Biotechnology Research, 1 (1) : 2-9.

Nasrudin, J. 2015. Efektivitas pemberian hormon ovaprim terhadap latensi pemijahan dan daya tetas telur ikan ele (Clarias sp). Skripsi. Program Studi Biologi. FMIPA Universitas PGRI Adibuana. Surabaya.

Ojutiku, R.O. 2008. Comparative survival and growth rate of Clarias gariepinus and Heteroclarias hathclings fed live and frozen Daphnia. Pakistan J of Nutr,7,4:527-529. ISN 1680-5194.

Sinjal, H. 2014. Ektifitas ovaprim terhadap lama waktu pemijahan, daya tetas telur dan sintasan larva ikan lele dumbo, Clarias gariepinus. Budidaya Perairan, 2(1):14-21.

Szabo, T ., 2003. Ovulation induction in northen pike Esox lucius L. using different GnRH analogues, ovaprim, dagin and carp pituitary. Aquacult. Res., 34: 479-486.

Szabo, T., C. Medgyasszay and L. Horvath, 2002. Ovulation induction in nase (Chondrostoma nasus, Cyprinidae) using pituitary extract or GnRH analogue combined with domperidone. Aquaculture, 203: 389-395.

Yaron, Z. 1995. Endocrine control of gametogenesis and spawning induction in the carp. Aquaculture, 129: 49-73.

Zhuo, Q, Y. Zhang., W. Huang., X. Liu., Y. Li., P. Zhu, D.. Lu. and H. Lin. 2011. Gonadotropin releasing hormone analogue multiple injection potentially accelearted testicular maturation of male yellow catfish (Pelteobagrus fluridruco Richardson) in captivity. Aquacul. Res. 42:1-14.

Zohar, Y.andC.C. Mylonas,2001. Endocrine manipulation of spawning induction in cultured fish from hormone to gene. Aquaculture, 197, 99-139.