Manufacture of Local Microorganism (MOL) from Vegetable Waste with Nutrition Source Supply VariationAbstract views: 406
Keywords:local microorganism; rice washing water; vegetable waste
AbstractLocal Microorganisms (MOL) is a bioactivator consisting of a collection of various kinds of microorganisms that function as agents for the decomposition of organic matter. Vegetable waste is a collection of various kinds of vegetables after being sorted because they are not suitable for sale. Almost all vegetable waste will undergo lactic acid fermentation carried out by lactic acid bacteria (LAB). Lactic acid bacteria (LAB) are gram-positive bacteria, do not form spores, are catalase negative, are resistant to acidic conditions, and are facultative anaerobes. The purpose of this study was to determine the correct composition of rice washing water and granulated sugar for the highest number of microbes in MOL. MOL optimization was carried out by means of anaerobic fermentation for 14 days. The results of this study indicate that the microbes contained in MOL are Bacillus subtillus, Bacillus cereus, Lactobacillus acidophilus, Spirillum, Streptoverticillium, and Leuconostoc mesenterousdes. The highest total population of microorganisms in variations in the composition of rice washing water and sugar is in sample C1 which is 91 x 105 Colonies/100 mL in MOL vegetable waste, which is 650 mL of rice washing water and 100 grams of sugar.
Adriani L, Indrayati N, U. H T, Mayasari N. Activity of Lactobacillus acidophillus and Bifidobacterium on Yogurt Quality and Its Inhibition on Helicobacter pylori, Journal of Bionatura, 2008, Vol. 10(2).
Alamsjah MA, Christiana RF, Subekti S. Effect of Fermentation of Seaweed Waste Gracilaria sp. With Bacillus subtilis Against Chlorophyceae Plankton Population, Journal of Fisheries and Marine Sciences, 2011, Vol. 3(2)
Aliya H, Maslakah N, Numrapi T, Buana AP, Hasri YN. Utilization of Lactic Acid from Fermentation of Cabbage Waste as a Wine and Strawberry Preservative. Bioeducation Journal of Biology Education, 2016, Vol. 8(2).
Antonius S, Agustiyani D. Effect of Biological Organic Fertilizer Containing Beneficial Microbes on Growth and Yield of Watermelon Plants and Soil Biochemical Properties in Field Experiments in Malinau-East Kalimantan. Berk Biological Research, 2011, Vol. 16(2).
Budiyani N, Soniari N, Sutari N. Analysis of the Quality of Solutions of Local Microorganisms (MOL) Banana Weevil. E-Journal of Trop Agroecotechnology, 2016, Vol. 5(1).
Hersoelistyorini, Wikanastri; Dewi SS. Identification of Probiotic Bacterial Proteins from Cabbage and Mustard Waste Based on Electrophoresis SDS-PAGE. Published Online, 2012.
Hidayah TM. Differences in the Chemical Quality of Cow's Milk Kefir, Soy Milk, And Red Bean Milk. Sainstech Innovation Journal, 2019, Vol. 2.
Indriani F, Utrisno E, Sumiyat S. Study of the Effect of Adding Fish Waste to the Process of Making Liquid Fertilizer from Cow Urine on Macro Nutrients (CNPK). Journal of Environmental Engineering, 2013, Vol. 2(2).
Irpan I, Caronge MW, Fadilah R. Water Mole Test of Siwalan (Borassus flabellifer) with the addition of various types of fruit based on the duration of fermentation. Journal of Agricultural Technology Education, 2018.
Khoiriyah, Ma’rifatul; Sutanto, Agus; Sulistiani WS. Effect of Indigenous Bacterial Consortia on the Quality of Cow Urine Liquid Fertilizer. Proceedings of the National Education Seminar, 2017.
Kunarso; Djoko H. The Role of Heterotrophic Bacteria in Marine Ecosystems, 1988.
Kusmiati; Malik A. Bacteriocin Activity of Leuconoctoc mesenteroides Pbacl Bacteria on Various Media, Health, 2002, Vol. 6 No.1.
Manullang RR, Rusmini R, Daryono D. Combination of Local Microorganisms as Compost Bioactivator. Tropical Forest Journal, 2018 Vol. 5(3).
Marsiningsih N, Suwastika A, Sutari N. Quality Analysis of MOL (Local Microorganisms) Solution Based on Tofu Dregs. E-Journal of Tropical Agroecotechnology, 2015, Vol. 4(3).
Moeksin R, Sari W, Eni. Making Bioethanol From Rice Washing Wastewater Using Enzymatic Hydrolysis And Fermentation Methods. Journal of Chemical Engineering, 2015, VOl. 21(1).
MP LW. EM4 And MOL . Microorganism Technology. BPP Jambi, 2015.
Nugroho, Herbudhi Cahyo; Amalia, Ulfah; Rianingsih L. Physicochemical Characteristics of Shredded Fish Meatballs With The Addition Of Transglutaminase At Different Concentrations. Journal of Fisheries Science and Technology, 2019, Vol. 1 No.2.
Nuraini A, Ibrahim R, Studi P, Hasil T, Diponegoro U, Merah G. The Effect of Addition of Different Concentrations of Carbohydrate Sources from Rice and Brown Sugar to the Quality of Red Tilapia Fish (Oreochromis Niloticus). Fisheries Saintek, 2014, Vol. 10(1).
Nurhadianty VCC. Introduction to Industrial-Scale Fermentation Technology UB Press, Malang, 2018.
Palupi NP. Chemical Characteristics of Compost With Decomposers of Local Microorganisms from Vegetable Waste, 2015, Vol. 40 (1).
Prayitno, S.H.; Widiyanto Dan Utama C. Use Of Waste Vegetable Extracts In Combination Rumen Fluid As A Starter On Total Fungi With Existence Molds And Yeasts. Anim Agric Journal, 2014, Vol. 3(4).
Purnama Wb. Glucose Antibacterial Activity Against Bacteria Staphylococcus aureus, Pseudomonas aeruginosa, bacillus subtilis, dan Escherichia coli, 2013.
Ruhnayat A. Utilization of Bio Fertilizers and Natural Fertilizers to Support Organic Cultivation of Pepper and Vanilla Plants. Published Online, 2007.
Sari, Dwi Riana; Santoso, Handoko; Zen S. The Effect of Types of Indigenous Bacterial Consortia Lcn on the Quality of Tofu Waste Liquid Fertilizer. Proceedings of the National Seminar on Education. Proceedings of the National Education Seminar. Published Online, 2017.
Sektiono, Antok Wahyu; Kajariyah, Siti Nur; Djauhari S. Test of Antagonism of Actinomycetes rhizhosphere and Root Endophytes of Chili Plants (Capsicum frutescens L.) Against the Fungi Colletotrichum capsici (Syd.) Bult Et Bisby. HPT, 2016, Vol. 4(1).
Sulistiyono. Determination of Carbohydrate Types By Qualitative Test Using Reagents on Instant Noodle Samples. Biology Journal. Published Online, 2010.
Sunarsih LE. Waste Management. Deepublish, Yogyakarta, 2018.
Supono. Environmental Management For Aquaculture. ISBN 978-602-6912-04-6. Published Online, 2015.
Suprihatin, Suci Perwitasari D. Making Lactic Acid From Cabbage Waste. Faculty of Technology Indonesia East Java. Published Online, 2010.
Suwarno S, Ratnani R, Hartati I. The Process of Making Invert Sugar From Sucrose With Citric Acid, Tartaric Acid And Hydrochloric Acid Catalysts. Unwahas Momentum Journal, 2015, Vol. 11(2).
Suwarno; Perwitasari; Dyah S. Utilization of Vegetable Waste Moles in the Compost Making Process. MIPA Journal, 2017, Vol. 40(1).
Syahrizal. Efektivitas Dosage of Em4 (Effective Microorganism) In Making Liquid Fertilizer From Organic Waste. Journal of Health Sciences Nasuwakes, 2015, Vol. 8 No.1.
Utama, Cs; Mulyanto A. The Potential of Vegetable Market Waste to Become a Fermentation Starter. Health Journal. Published Online, 2009.
Utama N. Growth And Yield Of Lettuce Plant (Lactuca Sativa) That Were Given Organic Chicken Manure Plus Some Bioactivators. Agricultural Empowerment Journal. Published Online, 2014.
W Amalia, Deasy; Widiyaningrum P. The Use of EM4 And MOL Tomato Waste As Bioactivators In Compost Making. Published Online, 2016.
Waluyo L. Waste Bioremediation. UMM Press, Malang, 2018.
Widowati, Tri Wardani; Malahayati N. Effect of Addition of Salt on Chemical and Microbiological Characteristics of Pickled Mustard (Brassica juncea) During Fermentation With Coconut Water Medium. National Seminar on Suboptimal Land. Published Online, 2016.
Wignyanto; Hidayat N. Bioindustri. Ub Press, Malang, 2017.
Wijayanto A, Lani A. Optimizing Vegetable Products Alternatives and On-Line-Based Marketing. Pambudi, 2018, Vol. 1(