AKTVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MENTIMUN (Cucumis sativus Linn) TERHADAP Salmonella typhi dan Bacillus cereus SECARA IN VITRO
DOI:
https://doi.org/10.36456/stigma.vol9.no01.a317Abstract
The objective of this studi was evaluate the antibacterial activity of Cucumber fruit against Salmonella typhidan Bacillus cereus in vitro. Ethanol extract of Cucumber fruit devided into 4 doses 100%, 75%, 50%, and
25% S. typhi dan B. cereus. Amoxicillin were used as positive control and aquadest as negative control. The
result this study showed antibacteria activity of ethanol extract Cucumber fruit inhibited the growth of
Salmonella typhi and Bacillus cereus. Antibacterial activity of Cucumber fruit 100% concentration of the
extract produce inhibited zone greatest is 7,7±0,92 mm included categorized intermediate. Whereas B.
cereus inhibited zone greatest is 2,6±0,39 mm, and dan included categorized weak.
Keywords:
Antibacterial activity, Salmonella typhi, Bacillus cereus, in vitro, Ethanol extract cucumber fruit
References
Aboaba, O.O., S.I. Smith., and F.O. Olude. 2006. Antibacterial Effect of Edible Plant Extract on Escherichia coli 0157:H7. Pakistan Journal of Nutrition. 5 (4): 325-327
Amrita, V., D. Sonal., R. Shalini. 2009. Antibacterial Effect of Herbs and Spices Extract on
Escherichia coli. Electronic Journal of Biology. Vol. 5(2): 40-44
Ariza, B.T.S., D.C. Mufida., N.N. Fatima., T.I. Hendrayati., T. Wahyudi., and Misnawi. 2014. In Vitro Antibacterial Activity of Cocoa Ethanolic Extract AgainstEscherichia coli. International Food Research Journal. 21(3): 935-940
Davis, W. W. dan T. R. Stout. 1971. Disc Plate Methods of Microbiological Antibiotic Assay. Applied Microbiology 22 (4): 666-670.
Das, J., A. Chowdhury., S.K. Biswas., U.K. Karmakar., S.R. Sharif., S.Z. Raihan., and M.A. Muhit. 2012. Cytotoxicity and Antifungal Ativities of Ethanolic and Chlorofrm Extracts of Cucumic sativus Linn (Cucurbitaceaae) Leaves and Stems. Res. J. Phytochem. ISSN 1819-3471
Gopalakrishnan, S.B., and T. Kalaiaras. 2014. Screening of Various Extracts of the Fruits of Cucumis sativus Linn. for Antimicrobial ctivity. Int. J. Res. Dev. Pharm. L. Sci.Vol. 3, No.5 : 1200-1205
Jenkins, C., and S.H.Gillespie. 2006. Salmonellaspp in Principles and Practice of Clinical Bacteriology Second Edition. (Ed) Stephen H. Gillespie dan Peter M. Hawkey. John Wiley & Sons Ltd
Johannessen, G.S., K.F. Eckner., N. Heiberg., M. Monshaugen., M. Begum, M. Økland., and H.R. Høgåsen. 2015. Occurrence of Escherichia coli, Campylobcter, Salmonella and Shiga-Toxin Producing E. coli in
Norwegian Primary Strawberry Production. Int. J. Environ. Res. Public Health. 12 : 6919-6932
Lubote, R., F. Shahada., and Athanasia Matemu. 2014. Prevalence of Salmonella spp. and Escherichia coli in Raw Milk Value Chain in Arusha, Tanzania. American Journal of Research Communication. Vol 2(9) : 1-13
Logan, N.A. and M.R. Díaz. 2006. Bacillus spp. and Related Genera in Principles and Practice of Clinical Bacteriology Second Edition. (Ed) Stephen H. Gillespie dan Peter M. Hawkey. John Wiley & Sons Ltd
Mamman, P.H., W.P. Mshelia., S.C. Susbatrus., and K.W. Sambo. 2013. Antibacterial Effects of Crude Extract of Azadirachta Indica Against Escherichia coli, Salmonella spp and Staphylococcus aureus. International Journal of Medicine and Medical Sciences. Vol. 5(1): 14-18
Muruganantham, N., S. Solomon., M.M. Senthamilselv. 2016. Antimicrobial Activity of Cucumis sativas (Cucumber) Flowers. Int. J. Pharm. Sci. Rev. Res., 36(1), No. 16: 97-100
RadušienÄ—, J., B. KarpaviÄienÄ—., and Ž. Stanius. 2012. Effect of External and Internal Factors on Secondary Metabolites Accumulation in st. John’s Worth. Botanica Lithuanica. 18(2): 101–108
Rahmawati, F., dan S.H. Bintari. 2014. Studi Aktivitas Antibakteri Sari Daun Binahong (Anredera cordifolia) terhadap Pertumbuhan Bacillus cereus dan Salmonella enteritidis. Unnes Journal of Life Science. 3 (2): 103-111
Senthilkumar, B., and G. Prabakaran. 2005. Multidrug Resistant salmonella typhi in Asymptomatic Typhoid Carriers among Food Handlers in Namakkal District, Tamil Nadu. Indian Journal of Medical Microbiology, 23 (2):92-94
Toy, T.S.S., B.S. Lampus., dan B.S.P. Hutagalung. 2015. Uji Daya Hambat Ekstrak Rumput Laut
Gracilaria sp Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal e-GiGi (eG), Volume 3, Nomor 1: 153-159
Winias, S., A. Retno., R. Magfiroh., Nasrulloh., M. Ryan., and R.P. Rahayu. 2011. Effect of Cynammyldehyde from Cinnamon Extract as A Natural Preservative Alternative to the Growth of Staphylococcus aureus Bacteria. Indoneisan Journal of Tropical and Infection. Disease. Vol 2. No.1 : 38-41.










