POTENSI EKSTRAK JAHE MERAH (Zingiberaceae officinale rosc)SEBAGAI ANTIMIKROBA DAN PENINGKAT KUALITAS SPERMATOZOA MENCIT YANG DIINFEKSI BAKTERI Staphylococcus aureus SECARA INTRA URETHRA

Abstract

Red Ginger (Zingiberaceae officinale rosc) is known to be used as an anti-microbial and
enhancing the quality of spermatozoa. This study aims to demonstrate of the red ginger extract
(Zingiberaceae officinale rosc)potential as an antimicrobial and the quality enhancer of spermatozoa
in laboratory rats injected by Staphylococcus aureus to its urethra. The red ginger was extracted by
ethanol. The sample of this research was the spermatozoa of 30 mice that were injected by
Staphylococcus aureus to its urethra. Potential test of red ginger extract on the laboratory rats
conducted by observing the spermatozoa’s motility, viability, morphology, the spermatozoa’s
concentration and the amount of spermatozoa leukocyte in the laboratory rats after the administration
of the red ginger extract for 35 days under the microscope. Antimicrobial activity test on
Staphylococcus aureus was done by culturing the spermatozoa of laboratory rats (in vivo) after
administering the red ginger extract for 35 days with total plate count method. The result of the study
showed that there were differences between negative control group of laboratory rats and positive
control group of laboratory rats (laboratory rats injected with Staphylococcus aureus intra urethra)motility (p = 0.000), viability (p = 0.000), morphology (p = 0.000), concentration (p = 0.000), and the
amount of leukocyte (p = 0.000). Whereas on the calculation of red ginger extract bacterial colonies
give the significant effects p <0.05 on the growth of S. aureus. Based on the results of this study, it can
be conclude that the red ginger has potential as an antimicrobial and it also can improve the quality of
spermatozoa in laboratory rats infected with S. aureus through its urethra.