Antibacterial Activity Test of Mahkota Dewa Fruit (Phaleria macrocarpa) Extract against Escherichia Coli Bacteria
DOI:
https://doi.org/10.36456/embrio.v15i1.6533Keywords:
Antibacterial, Phaleria macrocarpa, Escherichia Coli, Flavonoid.Abstract
Exposure to Escherichia coli during pregnancy may cause serious problems such as anemia, premature birth and an increased risk of inflammation of the lining of the brain in the fetus. Mahkota Dewa (Phaleria macrocarpa) is a medicinal plant that is widely known and used to treat allergies and chronic diseases. One of the compounds contained in Mahkota Dewa is flavonoids. There is certain mechanism of action of flavonoids in inhibiting bacteria namely by damaging the cytoplasmic cell wall in bacteria. This study aims to analyze the antibacterial properties of various flavonoid concentrations of Mahkota Dewa fruit (Phaleria macrocarpa) extract against Escherichia coli bacteria through a laboratory experimental study design by testing the antimicrobial potency of the well method, namely 10percent, 20percent, 40percent, 60percent, 80percent and 100percent. Statistical analysis using One-Way Anova Test showed a significant difference between changes in the concentration of flavonoids in Mahkota Dewa fruit extract and the inhibition zones towards the growth of Escherichia coli bacteria (p less than 0.05). Pearson correlation test showed a very strong relationship with a positive direction (r equals 0.874) which indicated that the higher the concentration of flavonoids in Mahkota dewa fruit extract, the stronger the antimicrobial effect. Based on the study findings, it can be concluded that the flavonoid extract in Mahkota Dewa fruit extract had an antimicrobial potential against Escherichia Coli in vitro.
References
Addo, H. A, S. Hussen, dan L. Chelkeba. (2018). Childhood Bacterial Meningitis: Antimicrobial Use Pattern and Treatment Outcomes: a Prospective Observational Study. - Panyliksikan Google. (n.d.). Retrieved on March 28, 2022,
Adelberg, Jawetz, & Melnick. 2017. Medical Microbiology, 27 ED, Jakarta. Penerbit Buku Kedokteran EGC. - Panyliksikan Google. (n.d.). Retrieved on March 28, 2022,
Adibi, S., Nordan, H., Ningsih, S.N, Kurnia, M., Rohiat, S. 2017. Aktivitas Antioksidan Dan Antibakteri Ekstrak Daun Strobilanthes crispus Bl (Keji Beling) ) Terhadap Staphylococcus Aureus Dan Escherichia Coli. Jurnal Pendidikan dan Ilmu Kimia: 1( 2): 148-154 . - Panyliksikan Google. (n.d.). Retrieved on March 28, 2022,
Ananda, N., Dwi Sulistyani, L., & Winiati Bachtiar, E. (2017). Pertimbangan Penggunaan Implan Gigi pada Lansia. Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva, 6(1), 47–55. https://doi.org/10.18196/di.6181 - Panyliksikan Google. (n.d.). Retrieved on March 28, 2022, DOI: https://doi.org/10.18196/di.6181
Anik Eko, dan Dinda zahriani. 2016. Isolasi dan Identifikasi Saponin pada Ekstrak Daun Mahkota Dewa dengan Ekstraksi Maserasi. Jurnal Sains. Vol.6 No.12 December 2016 - Panyliksikan Google. (n.d.). Retrieved on March 28, 2022.
Bruyand, M., Mariani-Kurkdjian, P., Hello, S. Le, King, L. A., Cauteren, D. Van, Lefevre, S., Gouali, M., da Silva, N. J., Mailles, A., Donguy, M. P., Loukiadis, E., Sergentet-Thevenot, D., Loirat, C., Bonacorsi, S., Weill, F. X., De Valk, H., Djeddi, D. D., Allard, L., Roullaud, S., … Vrillon, I. (2019). Paediatric haemolytic uraemic syndrome related to Shiga toxin-producing Escherichia coli, an overview of 10 years of surveillance in France, 2007 to 2016. Euro Surveill., 24(8), 1800068. https://doi.org/10.2807/1560-7917.es.2019.24.8.1800068 DOI: https://doi.org/10.2807/1560-7917.ES.2019.24.8.1800068
Cassini, A., Högberg, L. D., Plachouras, D., Quattrocchi, A., Hoxha, A., Simonsen, G. S., Colomb-Cotinat, M., Kretzschmar, M. E., Devleesschauwer, B., Cecchini, M., Ouakrim, D. A., Oliveira, T. C., Struelens, M. J., Suetens, C., Monnet, D. L., Strauss, R., Mertens, K., Struyf, T., Catry, B., … Hopkins, S. (2019). Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European economic area in 2015: a population-level modelling analysis. Lancet Infect. Dis., 19(1), 56–66. https://doi.org/10.1016/s1473-3099(18)30605-4 DOI: https://doi.org/10.1016/S1473-3099(18)30605-4
Clermont, O., Dixit, O. V. A., Vangchhia, B., Condamine, B., Dion, S., Bridier-Nahmias, A., Denamur, E., & Gordon, D. (2019). Characterization and rapid identification of phylogroup G in Escherichia coli, a lineage with high virulence and antibiotic resistance potential. Environ. Microbiol., 21(8), 3107–3117. https://doi.org/10.1111/1462-2920.14713 DOI: https://doi.org/10.1111/1462-2920.14713
Cordita, Raka Novadlu., 2017. Skripsi. Perbandingan... - Google Cendekia. (n.d.). Retrieved on March 30, 2022,
Denamur, E., Clermont, O., Bonacorsi, S., & Gordon, D. (2020). The population genetics of pathogenic Escherichia coli. Nature Reviews Microbiology 2020 19:1, 19(1), 37–54. https://doi.org/10.1038/s41579-020-0416-x DOI: https://doi.org/10.1038/s41579-020-0416-x
DEWI, A. (2020). Efektivitas Pupuk Organik Cair (POC) Dari Batang Pisang Terhadap Pertumbuhan Bayam Hijau (Amaranthus Sp.) Dan Bayam Merah (Alternanthera Ficoides) Secara. http://eprints.ums.ac.id/id/eprint/84604
Downing, N. S., Zhang, A. D., & Ross, J. S. (2017). Regulatory Review of New Therapeutic Agents — FDA versus EMA, 2011–2015. New England Journal of Medicine, 376(14), 1386–1387. https://doi.org/10.1056/NEJMC1700103 DOI: https://doi.org/10.1056/NEJMc1700103
Halden, R. U. (2016). Lessons Learned from Probing for Impacts of Triclosan and Triclocarban on Human Microbiomes. MSphere, 1(3). https://doi.org/10.1128/MSPHERE.00089-16 DOI: https://doi.org/10.1128/mSphere.00089-16
Lindsey, R. L., Garcia-Toledo, L., Fasulo, D., Gladney, L. M., & Strockbine, N. (2017). Multiplex polymerase chain reaction for identification of Escherichia coli, Escherichia albertii and Escherichia fergusonii. J. Microbiol. Methods, 140, 1–4. https://doi.org/10.1016/j.mimet.2017.06.005 DOI: https://doi.org/10.1016/j.mimet.2017.06.005
Norma Retnaningrum, D., Rahmawati, W., Rahasti, A., & Widyagama Husada Malang Jawa Timur, S. (2021). Effect Of Virgin Olive Oil (EVOO) On Placenta Weight and Fetal Weight in Preeclampsia Model Wistar Rats. Embrio : Jurnal Kebidanan, 13(2), 115–124. https://doi.org/10.36456/EMBRIO.V13I2.3699 DOI: https://doi.org/10.36456/embrio.v13i2.3699
Okzelia, S., Hendrati, D., and, N. I.-J. of nursing, & 2017, undefined. (n.d.). Isolasi dan Pemisahan Senyawa Alkaloid dari Buah Mahkota Dewa (Phaleria macrocarpa Boerl.) Dengan Metode Kromatografi Cair. Jnh.Stikesbanisaleh.Ac.Id. Retrieved on March 29, 2022, from https://jnh.stikesbanisaleh.ac.id/index.php/jnh/article/view/16 DOI: https://doi.org/10.25099/stkbs.010209175
Rahmawati, W., Norma Retnaningrum, D., Rahasti, A., Studi DIII Kebidanan Sekolah Tinggi Ilmu Kesehatan Widyagama Husada Malang, P., & Bersalin Rumah Sakit Umum Daerah Bangil Pasuruan, R. (2020). Pengaruh EVOO (Extra Virgin Olive Oil) Terhadap Tekanan Darah dan Kadar Protein Urin Pada Tikus Model Preeklamsia. DINAMIKA KESEHATAN: JURNAL KEBIDANAN DAN KEPERAWATAN, 11(2), 584–592. https://doi.org/10.33859/DKSM.V11I2.664
Rahmawati, W., & Retnaningrum, D. N. (2022). Antifungi Test of Red Spinning Extract (Amaranthus tricolor L.) on The Growth of Candida albicans. Embrio : Jurnal Kebidanan, 14(1), 69–75. https://doi.org/10.36456/EMBRIO.V14I1.4636 DOI: https://doi.org/10.36456/embrio.v14i1.4636
Suryani 2007 mahkota dewa - Penelusuran Google. (n.d.). Retrieved April 10, 2023, from https://www.google.com/search?q=suryani+2007+mahkota+dewa&ei=BHQzZL_jJsCQseMPmO-EsAk&ved=0ahUKEwi_9a7ooZ7-AhVASGwGHZg3AZYQ4dUDCA4&uact=5&oq=suryani+2007+mahkota+dewa&gs_lcp=Cgxnd3Mtd2l6LXNlcnAQAzIFCCEQoAEyBQghEKABOgkIABAHEB4QsAM6BggAEBYQHjoECCEQFToHCCEQoAEQCjoICCEQFhAeEB1KBAhBGAFQnQhYiiVgsihoAXAAeACAAZ0CiAHPDpIBBTIuOS4ymAEAoAEByAEBwAEB&sclient=gws-wiz-serp
Touchon, M., Perrin, A., De Sousa, J. A. M., Vangchhia, B., Burn, S., O’Brien, C. L., Denamur, E., Gordon, D., & Rocha, E. P. C. (2020). Phylogenetic background and habitat drive the genetic diversification of Escherichia coli. PLoS Genet., 16(6). https://doi.org/10.1371/journal.pgen.1008866 DOI: https://doi.org/10.1371/journal.pgen.1008866
Vihta, K. D., Stoesser, N., Llewelyn, M. J., Quan, T. P., Davies, T., Fawcett, N. J., Dunn, L., Jeffery, K., Butler, C. C., Hayward, G., Andersson, M., Morgan, M., Oakley, S., Mason, A., Hopkins, S., Wyllie, D. H., Crook, D. W., Wilcox, M. H., Johnson, A. P., … Walker, A. S. (2018). Trends over time in Escherichia coli bloodstream infections, urinary tract infections, and antibiotic susceptibilities in Oxfordshire, UK, 1998–2016: a study of electronic health records. Lancet Infect. Dis., 18(10), 1138–1149. https://doi.org/10.1016/s1473-3099(18)30353-0 DOI: https://doi.org/10.1016/S1473-3099(18)30353-0
Yusuf, M., Kurniawan, H., R Pahlevi, A. B., Budiman, C., & Arief, I. I. (2020). Nomor 1 edisi Januari-Maret. Jurnal Sain Peternakan Indonesia, 15. https://doi.org/10.31186/jspi.id.15.1.84-90 DOI: https://doi.org/10.31186/jspi.id.15.1.84-90
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Dwi Norma Retnaningrum, Wenny Rahmawati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
